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Currently, a sequence region suitable for in situ hybridization probe is quested in the open-reading frame or 3’UTR, and determined appropriately. However, transcriptome analysis based on RNA sequencing has revealed that various levels of exon expression are generated in a given gene. Therefore, in order to obtain a significant result in in situ hybridization, the determination of the probe region is of utmost importance. We propose the candidate regions for probe(underlined with green), based on the customer’s thought.